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OMNIfect Transfection Reagent

OMNIfect™ transfection reagent is an easy-to-use, cationic delivery reagent providing high efficiency transfection of plasmid DNA coupled with low cytotoxicity across a broad range of cells. OMNIfect has been tested across a variety of cell lines and has proven to successfully transfect both adherent and suspension cell lines. 
  • Efficient delivery – high transfection efficiency with or without serum
  • Low cytotoxicity gentle on sensitive cell lines
  • Broad range of cells tested – including neuronal, stem and suspension cells
  • Easy-to-use protocols
OMNIfect transfection reagent has been proven to successfully transfect a broad range of cell types including suspension cells and benchmarks favorably against the market leaders (Figure 1).

Figure 1: HEK-293, COS-1, HepG2, C2C12, CHO and Jurkat  cells were seeded in 12-well plates and transfected with 1µg luciferase plasmid according to the manufacture’s protocols.  At 48 hours after transfection luciferase expression was quantified (relative light units /mg total protein) from cell lysates.  Data are presented as values (+/- SEM) relative to OMNIfect transfection.

High transfection efficiency

OMNIfect Transfection Reagent has been proven to  transfect a broad range of cell types including suspension cells with superior efficiency. When tested against competitive transfection reagents OMNIfect transfection reagent benchmarks favorably against the market leaders. The table shown in the supporting data tab lists the cell lines in which OMNIfect has been shown to produce high transfection efficiency.

Figure 2. Graph showing cell viability after transfection using OMNIfect transfection  reagent in COS-1 cells compared with untreated cells as a control.

Minimal cytotoxicity

OMNIfect delivery reagent is very gentle on cells. Cytotoxicity was tested in COS-1 (Figure 2) cells and virtually no cell loss was observed. Cell viability following transfection was tested using  CellTiter 96® AQueous One Solution Cell Proliferation Assay according to the manufacturer’s instructions.  Data are presented as viability of OMNIfect treated cells relative to untreated cells.