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MGC premier ORF Clones

Gateway-adapted entry ORF Clones

MGC premier ORF Collections provide a comprehensive resource of fully annotated entry ORFs derived from collections generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (in collaboration with the Broad Institute) and the international ORFeome Collaboration (OC). The ORF clones are provided in Gateway adapted entry vectors for fast and convenient transfer to any compatible expression vector. Open Reading Frame (ORF) clones contain only the protein coding sequence allowing for the addition of fusion tags.
 
 
Product Highlights:
  • Gateway entry vectors – easily transfer to any compatible expression vector
  • Available with and without stop codons
  • Available as individual clones, gene family and pathway libraries, whole genome libraries and custom rearrays
  • Expression-ready cloning vectors available with multiple fusion tag options to create your expression-ready tagged ORF
  • Custom cloning and lentiviral packaging services available
     

Clonal Isolation and End-Sequencing Option

Want to confirm the identity of your ORF before shipping?  We can streak isolate a colony and end sequence it before delivery.  Select the end-sequenced format when choosing your ORF from FETCH my gene.  This risk free option ensures you start with a verified clonal culture.

Lentiviral ORF Clones - Expression ready, V5 tagged

MGC premier human lentiviral ORF collection was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). For added convenience the lentiviral ORF expression vector was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, puri­fication, co-immunoprecipitation, protein localization and FACS analysis.
  • Ready-to-express with a V5 tag
  • Perfect match to its parent accession.
  • Lentiviral particle formats available
  • Human genome
  • Blasticidin resistance marker may be used in combination with vectors using the more common puromycin marker
  • Custom cloning service available for mouse and rat species



MGC premier ORF Clones (Human, Mouse and Zebrafish)

Cat# Product Name Literature Price Buy
TOH7000 MGC premier Human Lentiviral ORF clone - Bacterial Glycerol Stock View Request Pricing Contact Distributor
TOH6003 MGC premier Human ORF clone - Bacterial Glycerol Stock View Request Pricing Contact Distributor
TOM6004 MGC premier Mouse ORF clone - Bacterial Glycerol Stock View Request Pricing Contact Distributor
TOZ6009 MGC premier Zebrafish ORF clone - Bacterial Glycerol Stock View Request Pricing Contact Distributor

MGC premier Expression-ready Empty Vectors for Cloning

Cat# Product Name Literature Price Buy
TCC1300 MGC premier Expression Vector (pCS6) - cloning vector View Request Pricing Contact Distributor
TCC1000 MGC premier Expression Vector (pTCN) - cloning vector View Request Pricing Contact Distributor
TCC2000 MGC premier Expression Vector (pTCP) - cloning vector View Request Pricing Contact Distributor
TOC1001 MGC premier tagged ORF Expression Vector (pCMV-Myc-N) - cloning vector View Request Pricing Contact Distributor
TOC1002 MGC premier tagged ORF Expression Vector (pCMV-Myc-C) - cloning vector View Request Pricing Contact Distributor
TOC1003 MGC premier tagged ORF Expression Vector (pCMV-DDK-N) - cloning vector View Request Pricing Contact Distributor
TOC1004 MGC premier tagged ORF Expression Vector (pCMV-DDK-C) - cloning vector View Request Pricing Contact Distributor
TOC1000 MGC premier Lentiviral ORF Expression Vector (pLX304) View Request Pricing Contact Distributor

Need help with cloning your ORF into an expression-ready vector or lentiviral packaging service? Are you looking for a mutant version of your ORF clone?