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CRISPR ssODN Templates


CRISPR ssODN Template

Genome editing precision using ssODN template with CRISPR/Cas9 system


Create efficient gene knockin or knockout by combining CRISPR-Cas9 with customized single-stranded oligodeoxynucleotides (ssODNs).
Create point mutations, modify sequence segments, insert small tags or add a restriction site into your region of interest.


Customer receives:
  • 3 top ranking gRNA lentiviral vectors targeting human, mouse, or rat genes*
  • Cas9 lentiviral expression vector*
  • Non-targeting negative control
  • ssODN template (10 µM Oligonucleotide Stock)

gRNA lentiviral vectors available as bacterial glycerol stock or viral particle format.
*Lentiviral vectors available with your choice of selectable marker





Figure 1: The lower oligo strand is the ssODN incorporating the T529I mutation with PAM sites changed and HindIII restriction site inserted.
PCR primer binding sites flank the alterations for later analysis.

 

How to Order:

Simply fill out the short form to request a quote. Our specialists will contact you about your request within 24 hours to confirm your pricing request.