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shERWOOD-UltramiR shRNA Reagents

Yeast Knockout Collections

In 1996 S. cerevisiae became the first eukaryotic genome to be fully sequenced enabling the creation of the first molecular-barcoded, genome-wide yeast knockout collection (1, 2).  The collection includes over 20,000 deletion strains corresponding to 5,916 genes (including 1,159 essential genes).  
Each strain has a complete start-codon to stop-codon deletion of an open reading frame (ORF) that is flanked by two molecular barcodes.  This collection has been used successfully in a number of screens and has been cited over 590 times (3).

Advantages of the collection include:
  • Molecular barcodes allow high throughput parallel screening of entire genome
  • Complete deletions that ensure no residual gene function
  • Homozygous and heterozygous formats ready for screening
  • Haploid collections can be crossed to cell based assay strains to expand screening options
The following collections are available as individual strains, custom rearrays and pooled screening libraries.
  • Yeast Homozygous Diploid Collection - Genome wide screening under various conditions
  • Yeast Heterozygous Diploid Collection - Screening for haploinsufficient genetic interactions
  • Yeast MAT-a or MAT-alpha Haploid Collections - Cross to other strains expanding options for screening assays
  • Yeast Essential Collection - Targeting genes essential for growth on rich media

 

Pooled Screening Libraries

Now available in a pooled format for simultaneous analysis of large numbers of deletion strains through selective growth conditions. Identification and quantitation of the deletions surviving selection can be determined by hybridizing the deletions to a microarray containing all the barcode oligos. The barcode oligos are unique to each deletion, allowing rapid, high-throughput identification of the deletions. Each Yeast Deletion Pool contains all the deletions within the strain, equally represented within the pool. Yeast Deletion Pools are supplied in ten tubes, each containing 500 µl of the pooled deletions.