/Products/CRISPR Genome Editing/transEDIT-dual CRISPR/Cas9 Expression Vectors

Cas9 endonuclease expression is a key component in performing efficient gene editing. The level of Cas9 endonuclease expression has been shown to affect the frequency of generating genome-edited clones. The efficiency of delivery and resulting expression of the targeting components vary widely between cell types. (See supporting data for more detail)

 

Cas9 Expression Vector transOMIC offers a variety of options for Cas9 expression vectors to optimize your gene editing experiment when paired with transEDIT CRISPR gRNA constructs targeting your gene of interest. Use FETCH my gene search tool to find transEDIT CRISPR gRNA target gene sets.


   

transEDIT CRISPR Cas9 Nuclease Expression Vectors

Cat# Product Name Literature Price Buy
TECC1001 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1002 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1003 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1004 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1005 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1006 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1007 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1008 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor

transEDIT CRISPR Cas9 Nickase Expression Vectors

Cat# Product Name Literature Price Buy
TECN1001 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1002 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1003 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1004 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor

Cas9 Nuclease and Nickase Expression Vectors

transEDIT™ Cas9 Nuclease and Nickase expression vectors offer options to optimize expression and selection of high Cas9 expressing cells in your target cell line. Tet-inducible Cas9 vectors are also available and developed to allow for more efficient control over Cas9 expression in your target cell line.

Highlights:

  • Transfect or transduce target cell lines with lentiviral Cas9 expression vectors- Transduction allows for efficient generation of Cas9 stable cell lines.

  • Multiple Selectable markers and Reporters - Blasticidin and/or puromycin to perform antibiotic selection selecting for Cas9 expressing cells. Co-selection can be performed with gRNA expression vectors for more efficient indel production. Fluorescent reporters  (ZsGreen or tRFP) in Cas9 vectors allow for FACS sorting of high expressing Cas9 cells.

  • Inducible Cas9 expression allows the creation of cell lines that limit constitutive expression of Cas9 which may reduce off target effects and may also be a necessary component when knockout of an essential gene is being considered.

  • Creation of stable cell lines with Cas9 or inducible Cas9 allows for more uniform expression of Cas9 which is desirable in the context of larger multiplexed screens.

  • Paired gRNA with Cas9 Nickase  for gene editing - Use Cas9 D10A mutant expressing vectors in combination with 2 gRNAs to produce efficient and specific gene editing (Ran et al., 2013).

Interested in gRNA and paired nickase strategy? Complete Form

Vector Specifications:

The pCLIP lentiviral Cas9 Nuclease/Nickase expression vectors are available with three promoter options (EFS, hCMV and TRE3G) driving expression of Cas9.
 

pCLIP-Nuclease
pCLIP-Nickase

Choice of marker or reporter
(Blasticidin, Puromycin, turboRFP
or ZsGreen)

pCLIP-Nuclease-TRE3G
pCLIP-Nickase-TRE3G

Choice of marker or reporter
(Blasticidin, Puromycin, turboRFP
or ZsGreen)