/Products/CRISPR Genome Editing/transEDIT-dual CRISPR/Combinatorial Knockout

Perform combinatorial knockouts with the transEDIT-dual vector system.

Powerful genome-editing tool!

The simultaneous knock out of two genes can help identify functional relationships between genes, which can lead to a better understanding of biological systems and can have direct implications for cancer therapeutic development as well as other therapeutic advancements.

The transEDIT-dual CRISPR arrayed library is available for targeting over 19,000 genes in the human genome. The vector system used for constructing the arrayed library (pCLIP-dual) was engineered to express two different gRNAs targeting the gene from one lentiviral vector.

transOMIC now offers the pCLIP-dual vector system for combinatorial knockouts, where one vector expresses two gRNAs, each targeting a separate gene. 

Simply provide us your two genes of interest and we will provide you with a combinatorial knockout kit to carry out your combinatorial knockout experiment.

Kit includes:
  • A pool of 15-25 sequence-verified pCLIP-dual constructs, where each construct contains a different combination of gRNA designs. Screening your cell line with multiple combinations of gRNA designs allows you to quickly identify the construct(s) with the most potent gRNA design pairing for knocking out your two genes of interest. The pool is available as lentiviral particles or plasmid DNA formats.
  • A sequencing primer is provided for sequencing your cells that show the most interesting phenotypes. Since each pCLIP-dual construct contains a unique molecular barcode, the identification of the pCLIP-dual construct responsible for your phenotype is easy to obtain.
  • Each sequence-verified pCLIP-dual construct included in the pool is also provided as an individual glycerol stock, so you have the most potent pCLIP-dual combinatorial construct targeting your two genes on hand for further experiments.
  • Negative control (Glycerol Stock or Lentiviral Pariticles)



Step 1:  Provide us your two genes to be targeted.
Learn more about the CRoatan algorithm.
Step 2: We will design gRNAs targeting each gene using the CRoatan algorithm

Showing example of pairing combinations.
Step 3:  We pair gRNAs for each gene into our pCLIP-dual vector.  Each gRNA pair has a unique barcode sequence for easy identification.

Packaged for 100ul of 1x107 TU/ml
Step 4:  All sequence-verified pCLIP-dual clones are pooled for lentiviral packaging.
Step 5:  Infect your cell line with the pool and observe for phenotype.

Example: Selection of interesting phenotypes
Step 6:  Select interesting phenotypes and sequence with the primer supplied with the kit.  Identify unique sequences in each sequencing read. Barcode sequences for each gRNA are provided in the kit.
Step 7:  Choose glycerol stocks from the interesting phenotypes for follow up experiments. transOMIC will store all the unique clones created in the kit and offer lentiviral packaging service upon request.