/Products/CRISPR Genome Editing/transEDIT-dual CRISPR/Documents



transEDIT-dual CRISPR Arrayed Library-Brochure

Technical Manual
transEDIT-dual Lentiviral gRNA (pCLIP-dual-SFFV-ZSGreen) Target Gene Sets - Technical Manual
transEDIT-dual Lentiviral gRNA (pCLIP-dual) CRISPR Screening Libraries-(Plasmid DNA and Viral Particles) - Technical Manual
transEDIT Cas9 Expressing Cell Line - Technical Manual

Erard, N., Knott S., & Hannon, G. Molecular Cell, 2017. "An arrayed CRISPR library for individual, combinatorial or multiplexed gene knockout"
Doench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nature Biotechnology, 34, 184-191, 2016.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744125/ (Free Access)
Doench, J. G. et al. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nature Biotechnology, 32, 1262-1267, 2014.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262738/ (Free Access)
Chari, R., Mali, P., Moosburner, M. & Church, G. M. Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nature Methods, 12, 823-826, 2015.
http://escholarship.org/uc/item/7qt804sf#page-1 (Free Access)
Bae, S., Kweon, J., Kim, H. S. & Kim, J. S. Microhomology-based choice of Cas9 nuclease target sites. Nature Methods, 11, 705-706, 2014.
https://www.researchgate.net/publication/263513198_Microhomology-based_choice_of_Cas9_nuclease_target_sites (Free Access)
Shi, J. et al. Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nature Biotechnology, 33, 661-667, 2015.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529991/ (Free Access)