/Products/CRISPR Genome Editing/CRISPR Cas9 reagents/Cas9 Expression Vectors and Cas9 Expressing Cell Lines

Cas9 Expression Vectors and Cas9 Expressing Cell Lines for More Efficient Genome Editing

Cas9 endonuclease expression is a key component in performing efficient gene editing. The level of Cas9 endonuclease expression has been shown to affect the frequency of generating genome-edited clones. The efficiency of delivery and resulting expression of the targeting components vary widely between cell types. (See supporting data for more detail)

 

Cas9 Expression Vectors transOMIC offers a variety of options for Cas9 expression vectors and Cas9 stable cell lines to optimize your gene editing experiment when paired with transEDIT CRISPR gRNA constructs targeting your gene of interest. Use FETCH my gene search tool to find transEDIT CRISPR gRNA target gene sets.


transEDIT CRISPR Cas9 Nuclease Expression Vectors

Cat# Product Name Literature Price Buy
TECC1001 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1002 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1003 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1004 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1005 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1006 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1007 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECC1008 transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor

transEDIT CRISPR Cas9 Nickase Expression Vectors

Cat# Product Name Literature Price Buy
TECN1001 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Puro) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1002 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Blast)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1003 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-ZsGreen)(Bacterial Glycerol Stock) View Request Pricing Contact Distributor
TECN1004 transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-tRFP) (Bacterial Glycerol Stock) View Request Pricing Contact Distributor

transEDIT CRISPR Cas9 Nuclease Expressing Cell Lines

Cat# Product Name Literature Price Buy
TCCL1001 Hepa 1-6 Cas9 Nuclease Cell Line 2 x 10^6 Cells/ml - Blasticidin View Request Pricing Contact Distributor
TCCL2001 Hepa 1-6 Cas9 Nuclease Cell Line 2 x 10^6 Cells/ml - Puromycin View Request Pricing Contact Distributor
TCCL1002 HEK293T Cas9 Nuclease Cell Line 2 x 10^6 Cells/ml - Blasticidin View Request Pricing Contact Distributor
TCCL2002 HEK293T Cas9 Nuclease Cell Line 2 x 10^6 Cells/ml - Puromycin View Request Pricing Contact Distributor

Cas9 Nuclease and Nickase Expression Vectors

transEDIT™ Cas9 Nuclease and Nickase expression vectors offer options to optimize expression and selection of high Cas9 expressing cells in your target cell line. Tet-inducible Cas9 vectors are also available and developed to allow for more efficient control over Cas9 expression in your target cell line.

Highlights:

  • Transfect or transduce target cell lines with lentiviral Cas9 expression vectors- Transduction allows for efficient generation of Cas9 stable cell lines.

  • Multiple Selectable markers and Reporters - Blasticidin and/or puromycin to perform antibiotic selection selecting for Cas9 expressing cells. Co-selection can be performed with gRNA expression vectors for more efficient indel production. Fluorescent reporters  (ZsGreen or tRFP) in Cas9 vectors allow for FACS sorting of high expressing Cas9 cells.

  • Inducible Cas9 expression allows the creation of cell lines that limit constitutive expression of Cas9 which may reduce off target effects and may also be a necessary component when knockout of an essential gene is being considered.

  • Creation of stable cell lines with Cas9 or inducible Cas9 allows for more uniform expression of Cas9 which is desirable in the context of larger multiplexed screens.

  • Paired gRNA with Cas9 Nickase  for gene editing - Use Cas9 D10A mutant expressing vectors in combination with 2 gRNAs to produce efficient and specific gene editing (Ran et al., 2013).

Interested in gRNA and paired nickase strategy? Complete Form

Vector Specifications:

The pCLIP lentiviral Cas9 Nuclease/Nickase expression vectors are available with three promoter options (EFS, hCMV and TRE3G) driving expression of Cas9.
 

pCLIP-Nuclease
pCLIP-Nickase

Choice of marker or reporter
(Blasticidin, Puromycin, turboRFP
or ZsGreen)

pCLIP-Nuclease-TRE3G
pCLIP-Nickase-TRE3G

Choice of marker or reporter
(Blasticidin, Puromycin, turboRFP
or ZsGreen)

Cas9 Expressing Cell Line Kit

transEDIT-dual gRNA constructs plus a validated Cas9-expressing cell line in a ready-to-use genome editing kit


CRISPR-Cas9 gene-editing technology is quickly becoming a staple in every molecular biology lab. One of the keys to a successful CRISPR experiment is hands-on experience with this new system. The transEDIT-dual Cas9 kit allows you to quickly get started on your experiment to give you the real-time experience using CRISPR-Cas9 and evaluate actual results. Once you gain the experience, then you can perform your experiment with the cell line of your interest or biological necessity.

Whether you need to quickly test a hypothesis or just want to get your hands wet with a CRISPR-Cas9 genome-editing experiment, you can start with an affordable ready-to-use kit.

The transEDIT-dual gRNA constructs will have a ZsGreen fluorescent reporter and puromycin selection and the Cas9-expressing cell line will have your choice of puromycin or blasticidin selection – allowing you to select for cells that have the correct combination of gRNA constructs and the Cas9 enzyme.


Kit includes:
  • 3 transEDIT-dual gRNA clones targeting your gene of interest
  • Negative control
  • Validated Cas9-expressing cell line

Transfection Workflow
Experimental workflow using Cas9 positive cells and
pCLIP-dual gRNA vectors in a transfection reaction.
Transduction Workflow
Experimental workflow using Cas9 positive cells and
pCLIP-dual gRNA vectors in a transduction reaction.
Preview Preview
Basic requirements and workflow for transfection of
pCLIP-dual gRNAs into Cas9 positive cells.
  • pCLIP-dual gRNA glycerol stocks
  • DNA extraction kit
  • Transfection reagent (such as OMNIfect)
  • Cell culture workflow
  • Cas9 positive cell line
  • Selection capabilities (antibiotic and/or fluorescence)
  • Analysis via phenotype or molecular assays
Basic requirements and workflow for transduction of
pCLIP-dual gRNAs into Cas9 positive cells.
  • pCLIP-dual gRNA glycerol stocks
  • DNA extraction kit
  • 2nd , 3rd or 4th generation lentiviral packaging system
  • Transfection reagent (such as OMNIfect)
  • Cell culture workflow to produce virus
  • Ability to harvest and concentrate virus if necessary
  • Cas9 positive cells
  • Selection capabilities (antibiotic and/or fluorescence)
  • Analysis via phenotype or molecular assays

How to Order

The instructions to order Cas9 expressing cell lines as a kit are shown below in 6 easy steps. The Cas9 expressing cell lines can be purchased separately in the above price grid.

 How to order CRISPR Cas9 Cell Lines