/Products/CRISPR Cas9 for Genome Editing/CRISPR Cas9 reagents/gRNA Design

transEDIT gRNA and Vector Design

Optimized gRNA designs produce efficient cleavage at the targeted site while limiting off-target effects.  transOMIC technologies uses the most current design algorithms to provide transEDIT™ gRNA designs with the highest ranked efficiency and lowest number of off-targets effects (Hsu et al. 2013, Wang et al. 2013). gRNA designs are provided as single gRNA for making double stranded breaks (DSBs) as well as paired gRNA for use with Cas9D10A (nickase) strategies.

transOMIC uses the following general guidelines for gRNA design and provides additional curation to select the top ranking gRNA:

(1) High efficiency of cleavage

  • Protospacer adjacent motif (PAM) - design sequences target 20 nucleotides of genomic sequence immediately upstream of the PAM sequence 5'-NGG
  • GC-content - between 45% and 80%
  • Nucleotide position effects - including the experimentally-determined effects of single and multiple mismatches on the targeting efficiency of off-target mismatches
  • Location within the gene -  target mutations closest to the start codon to ensure the best chance for loss of protein function

(2) Low off-target effects

  • Minimum possible homologous sites - select design with limited number of off-target sites in the genome
  • Evaluate off-target sites – penalize designs with off-target sites expected to be efficiently cleaved based on number and location of mismatches

Vector Specifications:

transEDIT gRNA expression vectors provide effective delivery to a wide range of cell types including non-dividing or post-mitotic cells.  Multiple selection options for use as a single-vector (All-in-one), in combination with transEDIT Cas9 nuclease vectors or in paired gRNA/nickase experiments. 

                transEDIT gRNA plus Cas9 Expression Vector

                         Convenience

  • Efficient delivery of gRNA and Cas9 in one vector
  • Single marker selection

                      transEDIT gRNA Expression Vector

               Modular for optimization

  • Control Cas9 expression in your target cell line (shown to be important for efficient knockout)
  • Complementary selection strategies for efficient co-expression with Cas9 or for paired gRNA/Cas9 Nickase strategy

Use to quickly find your gRNA Target Gene Set
Or

for paired gRNA and nickase options


 
 

transEDIT gRNA Cloning Vectors

Cat# Product Name Literature Price Buy
TELG1001 transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-Puro) View Request Pricing Contact Distributor
TELG1002 transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-Blast) View Request Pricing Contact Distributor
TELG1003 transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-ZsGreen) View Request Pricing Contact Distributor
TELG1004 transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-RFP) View Request Pricing Contact Distributor

transEDIT gRNA plus Cas9 Cloning Vectors

Cat# Product Name Literature Price Buy
TELA1001 transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-Puro) View Request Pricing Contact Distributor
TELA1002 transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-Blast) View Request Pricing Contact Distributor
TELA1003 transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-ZsGreen) View Request Pricing Contact Distributor
TELA1004 transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-RFP) View Request Pricing Contact Distributor

transEDIT gRNA Non-targeting Controls - Bacterial Glycerol Stock

Cat# Product Name Literature Price Buy
TELG1011 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-Puro) View Request Pricing Contact Distributor
TELG1012 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-Blast) View Request Pricing Contact Distributor
TELG1013 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-ZsGreen) View Request Pricing Contact Distributor
TELG1016 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-Blast) View Request Pricing Contact Distributor
TELG1015 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-Puro) View Request Pricing Contact Distributor
TELG1018 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-RFP) View Request Pricing Contact Distributor
TELG1017 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-ZsGreen) View Request Pricing Contact Distributor
TELG1014 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-RFP) View Request Pricing Contact Distributor

transEDIT gRNA Non-targeting Controls - Viral Particles

Cat# Product Name Literature Price Buy
TELGV1011 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-Puro) (Viral particles) View Request Pricing Contact Distributor
TELGV1012 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-Blast) (Viral particles) View Request Pricing Contact Distributor
TELGV1018 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-RFP)(Viral particles) View Request Pricing Contact Distributor
TELGV1013 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-ZsGreen) (Viral particles) View Request Pricing Contact Distributor
TELGV1014 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-RFP) (Viral particles) View Request Pricing Contact Distributor
TELGV1016 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-Blast) (Viral particles) View Request Pricing Contact Distributor
TELGV1017 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-ZsGreen)(Viral particles) View Request Pricing Contact Distributor
TELGV1015 transEDIT gRNA Non-targeting Control (pCLIP-gRNA-hCMV-Puro) (Viral particles) View Request Pricing Contact Distributor

transEDIT gRNA plus Cas9 Non-targeting Controls - Bacterial Glycerol Stock

Cat# Product Name Literature Price Buy
TELA1011 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Puro) View Request Pricing Contact Distributor
TELA1012 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Blast) View Request Pricing Contact Distributor
TELA1015 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-Puro) View Request Pricing Contact Distributor
TELA1016 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-Blast) View Request Pricing Contact Distributor
TELA1017 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-ZsGreen) View Request Pricing Contact Distributor
TELA1018 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-RFP) View Request Pricing Contact Distributor
TELA1013 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-ZsGreen) View Request Pricing Contact Distributor
TELA1014 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-RFP) View Request Pricing Contact Distributor

transEDIT gRNA plus Cas9 Non-targeting Controls - Viral Particles

Cat# Product Name Literature Price Buy
TELAV1011 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Puro) (Viral particles) View Request Pricing Contact Distributor
TELAV1012 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Blast) (Viral particles) View Request Pricing Contact Distributor
TELAV1013 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-ZsGreen) (Viral particles) View Request Pricing Contact Distributor
TELAV1015 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-Puro) (Viral particles) View Request Pricing Contact Distributor
TELAV1016 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-Blast) (Viral particles) View Request Pricing Contact Distributor
TELAV1017 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-ZsGreen) (Viral particles) View Request Pricing Contact Distributor
TELAV1014 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-RFP) (Viral particles) View Request Pricing Contact Distributor
TELAV1018 transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-hCMV-RFP) (Viral particles) View Request Pricing Contact Distributor