/Resource Pages/FAQs/FAQs/CRISPR FAQs


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Click the tabs below to find frequently asked questions related to your product of interest and general information.

Question: How do I grow a glycerol stock of my transEDIT-dual clone?

Answer:   transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines
Question: How do I grow a glycerol stock of my transEDIT pCLIP-ALL or pCLIP-gRNA (single) clone?

Answer:   transEDIT CRISPR All-in-one gRNA plus Cas9 for gene knockout and gene editing Click document to download printable guidelines
Question: How should I store my transEDIT-dual glycerol stock?

Answer:   transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines
Question: How should I store my transEDIT pCLIP-ALL or pCLIP-gRNA (single)  glycerol stock?

Answer:  transEDIT CRISPR All-in-one gRNA plus Cas9 for gene knockout and gene editing Click document to download printable guidelines
Question: How should I store lentiviral particles for my transEDIT-dual clone?

Answer:  transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines

Question: How should I store lentiviral particles for my transEDIT pCLIP-ALL or pCLIP-gRNA (single)  clone?

Answer:  for gene knockout and gene editing Click document to download printable guidelines

Question: How do I sequence confirm my transEDIT-dual gRNA clone?

Answer:  transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines

Question: How do I sequence confirm my transEDIT pCLIP-ALL or pCLIP-gRNA (single)  gRNA clone?

Answer:  for gene knockout and gene editing Click document to download printable guidelines

Question: Where can I find the vector map for my transEDIT-dual clone?

Answer:  transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines
OR    visit vector maps and sequences webpage

Question: Where can I find the vector map for my transEDIT pCLIP-ALL or pCLIP-gRNA (single)  clone?

Answer:  for gene knockout and gene editing Click document to download printable guidelines OR    visit vector maps and sequences webpage

Question: How do I produce lentiviral particles from my gRNA clone?

Answer:  Click document to download printable guidelines 

Question: What safety considerations are there for your lentiviral vectors?

Answer:  Click document to download printable guidelines 

Question: How do I determine an MOI before I begin my experiment and why is this necessary?

Answer:  Click document to download printable guidelines 

Question: How do I determine my puromycin kill curve before I begin my experiment and why is this necessary?

Answer:  Click document to download printable guidelines 

Question: How do I perform a relative transduction before I begin my experiment?

Answer:  Click document to download printable guidelines