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Click the tabs below to find frequently asked questions related to your product of interest and general information.

Question: How do I grow a glycerol stock of my shRNA clone?

Answer:    shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines
Question: How should I store my shRNA glycerol stock?

Answer:   shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines
Question: How should I store lentiviral particles for my shRNA clone?

Answer:  shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines
Question: How do I sequence confirm my shRNA clone?

Answer:  shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines
Question: Where can I find the vector map for my shRNA clone?

Answer:  shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines OR    visit vector maps and sequences webpage
Question: How do I produce lentiviral particles from my shRNA clone?

Answer:  shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines

Question: What safety considerations are there for your lentiviral vectors?

Answer:  Click document to download printable guidelines
 

Question: How do I determine an MOI before I begin my experiment and why is this necessary?

Answer:  Click document to download printable guidelines 

Question: How do I determine my puromycin kill curve before I begin my experiment and why is this necessary?

Answer:  Click document to download printable guidelines 

Question: How do I perform a relative transduction before I begin my experiment?

Answer:  Click document to download printable guidelines 
Question: How do I concentrate my viral particles ?

Answer:   transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines
Question: How do I perform NextGen sequencing for my shRNA pooled screening library?

Answer:    shERWOOD-UltramiR lentiviral shRNA for gene knockdown in RNAi experiments Click document to download printable guidelines