Barcoded, Doxycycline-Inducible Lentiviral ORF Expression Clones

Designed by and created for the Alex Kentsis lab at Memorial Sloan Kettering Cancer Center

Transomic works with academic labs across the globe to provide genomic tools that help researchers succeed with their research interests. Those tools can then be offered to the scientific community for continued discovery. Here we offer inducible lentiviral ORF clones prepared for the Kentsis lab at Memorial Sloan Kettering Cancer Center. These inducible ORFs are stem cell regulator candidates in Acute Myeloid Leukemia.

Overview

• ORFs represent 18 genes that are regulators of stem cells in acute myeloid leukemia

• Each ORF has a unique 25 bp barcode

• Unique barcodes allow for the analysis of ORF overexpression by identifying cell advantages or disadvantages under a specific experimental condition

• Doxycycline inducible vector with an mCherry fluorescent reporter

• The ORF clones can be used individually or pooled for use in a screen

Deliverable

• Available as glycerol stocks or ready-to-use lentiviral particles

Timeline

• 48 hours for standard glycerol stock clones. Two weeks for ready-to-use lentiviral particles

For citations:

• "Takao et al. Kentsis Submitted"

Annotated maps and sequences of vectors are available from SnapGene^®

Click to view detailed SnapGene^® vector maps and download free SnapGene viewer.