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Frequently Asked Questions

 

Click the tabs below to find frequently asked questions related to your product of interest and general information.

Question: How do I grow a glycerol stock of my CRISPR clone?

Answer:  Click document to download printable guidelines
Question: How should I store my CRISPR glycerol stock?

Answer:  Click document to download printable guidelines
Question: How should I store my CRISPR lentiviral particles?

Answer:  Click document to download printable guidelines
Question: How do I sequence confirm my transEDIT CRISPR clone?

Answer:  Click document to download printable guidelines
 
Question: Where can I find the vector map for my CRISPR clone?
 
Answer:   Visit vector maps and sequences webpage
 
Question: Where can I find the sequences for my CRISPR constructs?

Answer:   Visit order support webpage
 
Question: How do I determine my antibiotic kill curve before I begin my experiment?  Why is this necessary?
 
Answer:  for gene knockout and gene editing Click document to download printable guidelines
 
Question: How do I transfect my CRISPR and increase the transfection efficiency?

Answer:  transEDIT-dual CRISPR-Cas9 gene knockout and gene editing Click document to download printable guidelines
 
Question: How do I determine my relative transduction efficiency before I begin my experiment?  Why is this necessary?

Answer:  for gene knockout and gene editing Click document to download printable guidelines
 
Question: What safety considerations are there for your lentiviral vectors?
 
Answer:  Click document to download printable guidelines 
 
Question: How do I produce lentiviral particles from my CRISPR constructs?

Answer:  Click document to download printable guidelines 
 
Question: How do I concentrate my viral particles?
 
Answer:  Click document to download printable guidelines 
 
Question: How do I determine the MOI (multiplicity of infection) and why is this necessary?

Answer:  Click document to download printable guidelines 
 
Question: How do I perform NGS for my CRISPR pool?

Answer:   transEDIT single gRNA and All-in-one Pooled Screening Libraries
                transEDIT-dual gRNA Pooled Screening Libraries