Frequently Asked Questions

Click the tabs below to find frequently asked questions related to your product of interest and general information.

Question: How do I grow a glycerol stock of my CRISPR clone?

Answer: Click document to download printable guidelines

Question: How should I store my CRISPR glycerol stock?

Answer: Click document to download printable guidelines

Question: How should I store my CRISPR lentiviral particles?

Answer: Click document to download printable guidelines

Question: How do I sequence confirm my transEDIT CRISPR clone?

Answer: Click document to download printable guidelines

Question: Where can I find the vector map for my CRISPR clone?

Answer: Visit vector maps and sequences webpage

Question: Where can I find the sequences for my CRISPR constructs?

Answer: Visit order support webpage

Question: How do I determine my antibiotic kill curve before I begin my experiment? Why is this necessary?

Answer: Click document to download printable guidelines

Question: How do I transfect my CRISPR and increase the transfection efficiency?

Answer: Click document to download printable guidelines

Question: How do I determine my relative transduction efficiency before I begin my experiment? Why is this necessary?

Answer: Click document to download printable guidelines

Question: What safety considerations are there for your lentiviral vectors?

Answer: Click document to download printable guidelines

Question: How do I produce lentiviral particles from my CRISPR constructs?

Answer: Click document to download printable guidelines

Question: How do I concentrate my viral particles?

Answer: Click document to download printable guidelines

Question: How do I determine the MOI (multiplicity of infection) and why is this necessary?

Answer: Click document to download printable guidelines

Question: How do I perform NGS for my CRISPR pool?

Answer: transEDIT single gRNA and All-in-one Pooled Screening Libraries
transEDIT-dual gRNA Pooled Screening Libraries

Frequently Asked Questions

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Frequently Asked Questions

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*Last Name:

*Company/Institute:

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Job Title:

Research Area:

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