Frequently Asked Questions

Click the tabs below to find frequently asked questions related to your product of interest and general information.

Question: How do I grow a glycerol stock of my shRNA clone?

Answer: Click document to download printable guidelines

Question: How should I store my shRNA glycerol stock?

Answer: Click document to download printable guidelines

Question: How should I store my shRNA lentiviral particles?

Answer: Click document to download printable guidelines

Question: How do I sequence confirm my shRNA clone?

Answer: Click document to download printable guidelines

Question: Where can I find the vector map for my shRNA clone?

Answer: Visit vector maps and sequences webpage

Question: Where can I find the sequences for my shRNA constructs?

Answer: Visit order support webpage

Question: How do I determine my antibiotic kill curve before I begin my experiment? Why is this necessary?

Answer: Click document to download printable guidelines

Question: How do I transfect my shRNA and increase the transfection efficiency?

Answer: Click document to download printable guidelines

Question: How do I determine my relative transduction efficiency before I begin my experiment? Why is this necessary?

Answer: Click document to download printable guidelines

Question: What safety considerations are there for your lentiviral vectors?

Answer: Click document to download printable guidelines

Question: How do I produce lentiviral particles from my shRNA constructs?

Answer: Click document to download printable guidelines

Question: How do I concentrate my viral particles?

Answer: Click document to download printable guidelines

Question: How do I determine the MOI (multiplicity of infection) and why is this necessary?

Answer: Click document to download printable guidelines

Question: How do I perform NGS for my shRNA pool?

Answer: Click document to download printable guidelines