Choose from our in stock and ready to ship genome-wide pooled libraries or our made-to-order libraries in the lentiviral vector of your choice
Pre-made CRISPR Knockout pooled libraries
CRISPR dual gRNA knockout human whole genome pooled library
- Dual gRNA library targets over 18,000 human genes with 3-5 dual gRNA constructs per gene
- All gRNAs were designed with the CRoatan algorithm. Read the publication in Molecular Cell
- Dual gRNA library is qualified by NGS to ensure equal representation of gRNAs
- Vector expresses two gRNAs targeting the same gene, contains puromycin selection and ZsGreen expression
- Plasmid DNA ships in 2-4 business days, lentiviral packaging requires an additional 2 week
CRISPR Dual-gRNA Knockout Human Genome Pooled Library
Dual gRNA vector:
pCLIP-dual-SFFV-ZsGreen-Puromycin
Additional Information
E. coli Host Strain: STBL2
Bacterial Selection: Ampicillin or Carbenicillin
Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
CRISPR single gRNA knockout human whole genome pooled library
- Single gRNA library targets over 18,000 human genes with 4 different gRNA constructs per gene
- All gRNAs were designed with the CRoatan algorithm. Read the publication in Molecular Cell
- Each pooled library is qualified by NGS to ensure equal representation of gRNAs
- Choose vector with just gRNA or gRNA + Cas9 (All-in-One) expression
- Each vector type expresses one gRNA targeting the gene and contains puromycin selection marker
- Plasmid DNA ships in 2-4 business days, lentiviral packaging requires an additional 2 weeks
CRISPR Single-gRNA Knockout Human Genome Pooled Library
Single gRNA vector:
pCLIP-gRNA-EFS-Puromycin
Additional Information
E. coli Host Strain: STBL2
Bacterial Selection: Ampicillin or Carbenicillin
Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
CRISPR Single-gRNA Knockout Human Genome Pooled Library
gRNA + Cas9 (All-in-One) vector:
pCLIP-ALL-EFS-Puromycin
Additional Information
E. coli Host Strain: STBL2
Bacterial Selection: Ampicillin or Carbenicillin
Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Made-to-Order CRISPR Knockout Pooled Libraries
CRISPR single gRNA knockout human whole genome pooled library
- Single gRNA library targets over 18,000 human genes with 4 different gRNA constructs per gene
- All gRNAs were designed with the CRoatan algorithm. Read the publication in Molecular Cell
- Each pooled library is qualified by NGS to ensure equal representation of gRNAs
- Choose vector with just gRNA or gRNA + Cas9 (All-in-One) expression
- Choose a vector that is the appropriate combination of promoter, antibiotic selection marker, and fluorescent reporter
- Plasmid DNA ships in 4-6 weeks, lentiviral packaging requires an additional 2 weeks
CRISPR Knockout Human Genome Pooled Library
Single gRNA
SELECT :
Format
Promoter
Selection Marker
Fluorescent Reporter
Additional Information
E. coli Host Strain: STBL2
Bacterial Selection: Ampicillin or Carbenicillin
Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
CRISPR Knockout Human Genome Pooled Library
gRNA + Cas9 (All-in-One)
SELECT :
Format
Promoter
Marker/Reporter
Additional Information
E. coli Host Strain: STBL2
Bacterial Selection: Ampicillin or Carbenicillin
Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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Related Products and Services
Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique gRNA constructs plus a negative control. All gRNA constructs are 100% sequence-confirmed before shipping.
Transomic offers custom CRISPR pooled libraries for screening projects and genome editing experiments. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request (Broad-Calabrese, Broad-Brie, Broad-Gouda, Broad-Brunello, Weissman, Root, Doench…).
Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
Lentiviral Cas9 expression vectors can be used for creating your Cas9 stable cell lines and cell engineering experiments.
Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.
CRISPR Single gRNA Controls
CRISPR Dual gRNA Controls
View CRISPR-Cas9 Product Support
- Vector Maps - View all CRISPR related vector maps - Powered by SnapGene
- Product Guides - View guides using Transomic CRISPR products
- Publications - View publications relating to CRISPR products
- FAQs - View Frequently Asked Questions related to CRISPR products
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