Human Amplified gRNA Oligo Pool Library Construction Kit

Budget-Friendly Solution for creating a Human Whole Genome CRISPR gRNA Knockout Library for Screening

To help offset the high expense of performing whole genome CRISPR screening experiments, Transomic offers our high-quality, whole-genome, amplified CRISPR gRNA oligo pool and cloning vector kit at budget-friendly pricing. Using the two components, pool assembly and viral packaging can then be performed in your lab where you have complete control of the quality benchmarks that are important for your research experiment. Simply choose the cloning vector that suits your needs. We deliver an amplified, whole genome gRNA oligo pool and cloning vector along with detailed protocols for cloning and lentiviral packaging. We make it simple to create your customized CRISPR gRNA knockout library.



  • Cost-effective solution to create a customized, genome-scale human CRISPR gRNA library for knockout
  • gRNA oligo pool has been amplified with high efficiency saving you time and effort
  • Amplified gRNA oligo pool goes through rigorous quality control to ensure accuracy and uniformity of gRNA representation
  • Allows for complete control of your QC benchmarks specific to your experiment
  • Whole genome Human (Croatan gRNA designs) oligo pool targets over 18,000 genes with 4 gRNAs per gene
  • Cloning and lentiviral packaging protocols available to ensure your success


  • Ready-to-use cloning vector of your choice
  • Ready-to-use amplified whole genome gRNA oligo pool
  • QC reports for cloning vector and amplified gRNA pool


  •  Typically ships within 2-4 business days


$4,885 - Price includes the following
  • 1 Vial (125 mL): PCR amplified oligo pool- Prepared for Gibson Assembly Reaction (15 ng/ mL)
  • 1 Vial (100 mL) Vector Backbone- Prepared by restriction digest and CIP treatment, column purified (200 ng/ mL)

Oligo Pool Library Construction Workflow

Oligo Pool Whole Genome Library Construction Kit


Human Whole Genome Amplified gRNA Oligo Pool and Cloning Vector
Single gRNA
Selection Marker
Fluorescent Reporter

Human Whole Genome Amplified gRNA Oligo Pool and Cloning Vector
gRNA + Cas9 (ALL-in-One)

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CRISPR Oligo Library Construction Kit


Related Products and Services

Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique gRNA constructs  plus a negative control. All gRNA constructs are 100% sequence-confirmed before shipping.
Transomic offers custom CRISPR pooled libraries for screening projects and genome editing experiments. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request (Broad-Calabrese, Broad-Brie, Broad-Gouda, Broad-Brunello, Weissman, Root, Doench…).
Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.  This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
Lentiviral Cas9 expression vectors can be used for creating your Cas9 stable cell lines and cell engineering experiments.
Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.


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  • Publications - View publications relating to CRISPR products
  • FAQs - View Frequently Asked Questions related to CRISPR products