Cas9 Expression Vectors

Choose the ideal lentiviral vector for your Cas9 stable cell line

Cas9 lentiviral expression vectors can be used to efficiently transduce mammalian cell lines to engineer gene knockout stable cell lines. The Cas9 endonuclease, when programmed with a single gRNA, leads to a DNA double-strand break (DSB) at a desired genomic location. The cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to mend the targeted DSB. Choose the optimal promoter, selection marker, and fluorescent reporter for your CRISPR knockout experiment.

Deliverable

Glycerol stock for the generation of an unlimited supply of Cas9 plasmid DNA
Ready-to-use lentiviral particles
Plasmid DNA

Timeline

Glycerol stock format ships within 48 hours of placing order
Lentiviral particles ship within 2-3 weeks of placing order

Price

Glycerol stocks for $645

Available Cas9 expression vectors

pCLIP-Cas9-Nuclease

dCas9 Expression Vectors (dCas9-VPR and dCas9-KRAB) also available

Select your promoter and marker/reporter of choice and add to cart to purchase. Delivered as bacterial glycerol stocks.

Contact us at info@transomic.com for lentiviral particles or plasmid DNA quote.

transEDIT CRISPR Cas9 Nuclease Expression Vector

SELECT :

Promoter

Marker/Reporter

transEDIT CRISPR Cas9 Nuclease Expression Vector $645.00

Related Products and Services

CRISPR Target Gene Sets

Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique gRNA constructs plus a negative control. All gRNA constructs are 100% sequence-confirmed before shipping.

Custom Screening Pools

Transomic offers custom CRISPR pooled libraries for screening projects and genome editing experiments. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request (Broad-Calabrese, Broad-Brie, Broad-Gouda, Broad-Brunello, Weissman, Root, Doench…).

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

Custom CRISPR Vector Construction Service

Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.