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CRISPR Dual Cut gRNA
 


transEDIT-dual CRISPR Lentiviral Reagents


Dual gRNA expression for superior knockout efficacy


Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

 

transEDIT-dual gRNAs were developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis. The dual gRNAs are provided in a single vector to be used with Cas9 nuclease expression vectors.

 


 
transEDIT-dual CRISPR - two gRNAs in one lentiviral vector

 



Available vectors for your gene of interest: (Gene Knockout)


pCLIP-Dual

transEDIT-dual CRISPR vector map - pCLIP-dual gRNA

 

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