transEDIT-dual CRISPR Lentiviral Reagents
Dual gRNA expression for superior knockout efficacy
Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.
transEDIT-dual gRNAs were developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis. The dual gRNAs are provided in a single vector to be used with Cas9 nuclease expression vectors.
Available vectors for your gene of interest: (Gene Knockout)