Dual gRNA expression for superior knockout efficacy
Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.
The transEDIT-dual CRISPR-Cas9 Whole Genome Arrayed Library was developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis.
CRoatan algorithm gRNA designs result in superior knockout efficiency (read publication)
Two independent gRNAs in the same lentiviral vector further enhance for potent knockout
Unique barcode sequence identifier enables downstream analysis
All clones are sequence-verified
The transEDIT-dual CRISPR-Cas9 Whole Genome Arrayed Library targets over 19,000 genes in the human genome and is provided in 96-well plates as bacterial glycerol stocks.
Sequence-verified arrayed libraries are ideal resources for core labs
Contact us to request a quote
Fill out the short form to request a quote