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transEDIT-dual CRISPR Whole Genome Arrayed Library  


Dual gRNA expression for superior knockout efficacy

Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

 

transEDIT-dual CRISPR - two gRNAs

The transEDIT-dual  CRISPR-Cas9 Whole Genome Arrayed Library was developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis.
 

  • CRoatan algorithm gRNA designs result in superior knockout efficiency (read publication)

  • Two independent gRNAs in the same lentiviral vector further enhance for potent knockout

  • Unique barcode sequence identifier enables downstream analysis

  • All clones are sequence-verified


The transEDIT-dual CRISPR-Cas9 Whole Genome Arrayed Library targets over 19,000 genes in the human genome and is provided in 96-well plates as bacterial glycerol stocks.

 

Sequence-verified arrayed libraries are ideal resources for core labs

 

transEDIT-dual CRISPR Whole Genome Arrayed Library

 



Available vector:

pCLIP-Dual

transEDIT-dual CRISPR vector map - two gRNAs


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