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CRISPR Single Cut gRNA


transEDIT CRISPR-Cas9 Lentiviral Reagents

Optimized gRNA designs, versatile expression vectors for efficient gene editing
 

Fully functional CRISPR-Cas9 enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

 

transEDIT CRISPR-Cas9 lentiviral reagents include a range of lentiviral expression vectors expressing guide RNA (gRNA) or gRNA+Cas9. The sgRNA expression vectors are designed to select for high gene-editing efficiency using direct indicators of CRISPR-Cas9 expression from fluorescent reporters or selection markers. Choice of promoter, as well as efficient delivery as bacterial glycerol stock or lentiviral particles.
 


Schematic representation of RNA-guided double-stranded DNA cleavage by CRISPR-Cas9 using programmable guide RNA
Schematic representation of RNA-guided double-stranded
DNA cleavage by CRISPR-Cas9 using programmable guide RNA.

 

 

Available CRISPR vectors for your gene of interest: (Gene Knockout)


pCLIP-gRNA
transEDIT CRISPR gRNA vector map

pCLIP-All-In-One (gRNA+Cas9)
transEDIT CRISPR gRNA vector map - All-in-one (gRNA plus Cas9)

transEDIT CRISPR-Cas9 Supporting Data
CRISPR-Cas9 FAQs
CRISPR-Cas9 Publications
transEDIT Single gRNA Non-targeting Controls
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