CRISPR Single Cut gRNA

transEDIT CRISPR-Cas9 Lentiviral Reagents

Fully functional CRISPR-Cas9 enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

transEDIT CRISPR-Cas9 lentiviral reagents include a range of lentiviral expression vectors expressing guide RNA (gRNA) or gRNA+Cas9. The sgRNA expression vectors are designed to select for high gene-editing efficiency using direct indicators of CRISPR-Cas9 expression from fluorescent reporters or selection markers. Choice of promoter, as well as efficient delivery as bacterial glycerol stock or lentiviral particles.
 

• CRoatan algorithm gRNA designs result in superior knockout efficiency (Read publication: "An arrayed CRISPR library for individual, combinatorial and multiplexed gene knockout")
• CRISPR Target Gene Sets - 3 gRNA constructs targeting your gene of interest and a negative control
• Your choice of gRNA or gRNA+Cas9 lentiviral vectors
• Choose from bacterial glycerol stock or lentiviral particle formats
• All clones are sequence-verified!
• Custom CRISPR pooled screening libraries are available (Go to CRISPR libraries)

Schematic representation of RNA-guided double-stranded
DNA cleavage by CRISPR-Cas9 using programmable guide RNA.
 

Available CRISPR gRNA vectors for your gene of interest: (Gene Knockout)

pCLIP-All-In-One (gRNA+Cas9)