transEDIT CRISPR-Cas9 Lentiviral Reagents
Optimized gRNA designs, versatile expression vectors for efficient gene editing
Fully functional CRISPR-Cas9 enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.
transEDIT CRISPR-Cas9 lentiviral reagents include a range of lentiviral expression vectors expressing guide RNA (gRNA) or gRNA+Cas9. The sgRNA expression vectors are designed to select for high gene-editing efficiency using direct indicators of CRISPR-Cas9 expression from fluorescent reporters or selection markers. Choice of promoter, as well as efficient delivery as bacterial glycerol stock or lentiviral particles.
Schematic representation of RNA-guided double-stranded
DNA cleavage by CRISPR-Cas9 using programmable guide RNA.
Available CRISPR vectors for your gene of interest: (Gene Knockout)