Catalytically Inactive Cas9 Expression Vectors (dCas9)

Choose the ideal lentiviral vector for your dCas9 stable cell line

Catalytically deactivated, or dead Cas9 (dCas9) expression is a key component in performing efficient CRISPR activation (CRISPRa) or CRISPR interference (CRISPRi) experiments.  Cas9 has been modified to remove the nuclease activity but to keep the highly-specific genomic DNA targeting capability with the help of guide RNA.  The deactivated Cas9 (dCas9) for CRISPRa is fused to a series of transcription activators (VP64, p65 and Rta) to create a dCas9-VPR complex which, paired with specific gRNAs, upregulates gene expression. For CRISPRi, the dCas9 is fused to transcriptional repressor peptide, KRAB, to disrupt gene expression when paired with specific gRNAs.


  • Glycerol stock for the generation of an unlimited supply of dCas9 plasmid DNA
  • Ready-to-use lentiviral particles
  • Plasmid DNA

  • Glycerol stock format ships within 48 hours of placing order
  • Lentiviral particles ship within 2-3 weeks of placing order

  • Glycerol stocks for $585


Available dCas9-VPR expression vectors for CRISPR activation


Available dCas9-KRAB expression vectors for CRISPR interference


Cas9 Expression Vectors also available

Select your promoter and marker/reporter of choice and add to cart to purchase. Delivered as bacterial glycerol stocks.


Contact us at for lentiviral particles or plasmid DNA quote.

transACTIVE CRISPR dCas9-VPR Nuclease Expression Vector


transINTERFERE CRISPR dCas9-KRAB Expression Vector


Related Products and Services

Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique gRNA constructs  plus a negative control. All gRNA constructs are 100% sequence-confirmed before shipping.
Transomic offers custom CRISPR pooled libraries for screening projects and genome editing experiments. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request (Broad-Calabrese, Broad-Brie, Broad-Gouda, Broad-Brunello, Weissman, Root, Doench…).
Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.  This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.


CRISPR-Cas9 publications
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