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Catalytically Inactive Cas9 Expression Vectors (dCas9)  


Catalytically deactivated, or dead Cas9 (dCas9) expression is a key component in performing efficient CRISPR activation (CRISPRa) or CRISPR interference (CRISPRi) experiments.  Cas9 has been modified to remove the nuclease activity but to keep the highly-specific genomic DNA targeting capability with the help of guide RNA.  The deactivated Cas9 (dCas9) for CRISPRa is fused to a series of transcription activators (VP64, p65 and Rta) to create a dCas9-VPR complex which, paired with specific gRNAs, upregulates gene expression. For CRISPRi, the dCas9 is fused to transcriptional repressor peptide, KRAB, to disrupt gene expression when paired with specific gRNAs. 

Transomic offers a variety of options for dCas9 expression vectors to optimize your CRISPRa or CRISPRi experiments when paired with our transACTIVE CRISPR or transINTERFERE CRISPR target gene sets targeting your gene of interest.


Available dCas9-VPR expression vectors for activation

pACT-dCas9-VPR


 
 
Available dCas9-KRAB expression vectors for interference

pINT-dCas9-KRAB


 

Select your promoter and marker/reporter of choice and add to cart to purchase. Available as bacterial glycerol stocks.


Contact us at info@transomic.com for a viral particle quote.

transACTIVE CRISPR dCas9-VPR Nuclease Expression Vector

SELECT :
Promoter
Marker/Reporter

transINTERFERE CRISPR dCas9-KRAB Expression Vector

SELECT :
Promoter
Marker/Reporter