Catalytically Inactive Cas9 Expression Vectors (dCas9)

Choose the ideal lentiviral vector for your dCas9 stable cell line

Catalytically deactivated, or dead Cas9 (dCas9) expression is a key component in performing efficient CRISPR activation (CRISPRa) or CRISPR interference (CRISPRi) experiments. Cas9 has been modified to remove the nuclease activity but to keep the highly-specific genomic DNA targeting capability with the help of guide RNA. The deactivated Cas9 (dCas9) for CRISPRa is fused to a series of transcription activators (VP64, p65 and Rta) to create a dCas9-VPR complex which, paired with specific gRNAs, upregulates gene expression. For CRISPRi, the dCas9 is fused to transcriptional repressor peptide, KRAB, to disrupt gene expression when paired with specific gRNAs.

Deliverable

Glycerol stock for the generation of an unlimited supply of dCas9 plasmid DNA
Ready-to-use lentiviral particles
Plasmid DNA

Timeline

Glycerol stock format ships within 48 hours of placing order
Lentiviral particles ship within 2-3 weeks of placing order

Price

Glycerol stocks for $585

Available dCas9-VPR expression vectors for CRISPR activation

pACT-dCas9-VPR

Available dCas9-KRAB expression vectors for CRISPR interference

pINT-dCas9-KRAB

Cas9 Expression Vectors also available

Select your promoter and marker/reporter of choice and add to cart to purchase. Delivered as bacterial glycerol stocks.

Contact us at info@transomic.com for lentiviral particles or plasmid DNA quote.

transACTIVE CRISPR dCas9-VPR Nuclease Expression Vector

SELECT :

Promoter

Marker/Reporter

transACTIVE CRISPR dCas9-VPR Nuclease Expression Vector $645.00

transINTERFERE CRISPR dCas9-KRAB Expression Vector

SELECT :

Promoter

Marker/Reporter

transINTERFERE CRISPR dCas9-KRAB Expression Vector $645.00

Related Products and Services

CRISPR Target Gene Sets

Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique gRNA constructs plus a negative control. All gRNA constructs are 100% sequence-confirmed before shipping.

Custom Screening Pools

Transomic offers custom CRISPR pooled libraries for screening projects and genome editing experiments. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request (Broad-Calabrese, Broad-Brie, Broad-Gouda, Broad-Brunello, Weissman, Root, Doench…).

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

Custom CRISPR Vector Construction Service

Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.