Custom CRISPR Pooled Screening Libraries

Choose constructs from our genome-wide arrayed CRISPR library or chip-based pools for complete flexibility

With an extensive catalog of gRNA lentiviral vectors to choose from, Transomic offers complete flexibility when planning your custom CRISPR pooled screening library. Share your target gene list with us and we will use our CRoatan algorithm to design gRNAs targeting each gene. Or if preferred, share your gRNA designs (Weissman, Calabrese, Brunello, Doench…) to incorporate into the screening pool.

Target a small list of genes or whole genome
CRISPR knockout, activation, and interference are available
QC is based on Next-Gen Sequencing to ensure gRNA representation and equal distribution

Workflow:

Select the proper gRNA lentiviral cloning vector or provide us with your lentiviral vector
Share your gene list and the number of gRNAs needed for targeting each gene
The CRoatan algorithm is used to design gRNAs, or send your list of gRNA designs to synthesize
Oligos are synthesized and cloned into your choice of lentiviral vector
NGS is performed to ensure equal representation of gRNAs in your CRISPR screening pool
CRISPR whole genome arrayed library available

Deliverable:

Plasmid DNA (Maxiprep or Gigaprep) or ready-to-use lentiviral particles
Certificate of Analysis showing gRNA distribution according to Next-Gen Sequencing results

Timeline:

As few as 6 weeks for plasmid DNA. Add an additional 2 weeks for lentiviral particles

Retroviral vectors are also available

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