Custom CRISPR Pooled Screening Libraries


Choose constructs from our genome-wide arrayed CRISPR library or chip-based pools for complete flexibility
 

With an extensive catalog of gRNA lentiviral vectors to choose from, Transomic offers complete flexibility when planning your custom CRISPR pooled screening library. Share your target gene list with us and we will use our CRoatan algorithm to design gRNAs targeting each gene. Or if preferred, share your gRNA designs (Weissman, Calabrese, Brunello, Doench…) to incorporate into the screening pool.

  • Target a small list of genes or whole genome
  • CRISPR knockout, activation, and interference are available
  • QC is based on Next-Gen Sequencing to ensure gRNA representation and equal distribution


Workflow:

  • Select the proper gRNA lentiviral cloning vector or provide us with your lentiviral vector
  • Share your gene list and the number of gRNAs needed for targeting each gene
  • The CRoatan algorithm is used to design gRNAs, or send your list of gRNA designs to synthesize
  • Oligos are synthesized and cloned into your choice of lentiviral vector
  • NGS is performed to ensure equal representation of gRNAs in your CRISPR screening pool
  • CRISPR whole genome arrayed library available
 


Deliverable:

  • Plasmid DNA (Maxiprep or Gigaprep) or ready-to-use lentiviral particles
  • Certificate of Analysis showing gRNA distribution according to Next-Gen Sequencing results


Timeline:

  • As few as 6 weeks for plasmid DNA. Add an additional 2 weeks for lentiviral particles


Retroviral vectors are also available

 
 

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