Custom shRNA Arrayed Libraries


Choose constructs from our genome-wide arrayed shRNA library or chip-based pools for complete flexibility


Let Transomic build your custom shRNA arrays using any of our pZIP lentiviral vectors. Share your target gene list with us and we will use our shERWOOD algorithm to design shRNAs targeting each gene. Or if preferred, share your shRNA designs for cloning into our lentiviral vector, or a lentiviral vector that you provide to us. Each clone is sequence-verified and arrayed into 96-well plates. We can deliver glycerol stocks, plasmid DNA, and/or ready-to-use lentiviral particles in 96-well plates.

 


Workflow:

  • Select the appropriate pZIP lentiviral vector or provide us with your lentiviral vector
  • Share your gene list and the number of shRNAs needed for targeting each gene
  • The shERWOOD algorithm is used to design shRNAs. Alternatively, we can use your shRNA designs
  • Oligos are synthesized and cloned into your choice of lentiviral vector
  • Each clone is sequence-verified and arrayed into 96 well plates


Deliverable:

  • 96 well plates of glycerol stocks, plasmid DNA, and/or ready-to-use lentiviral particles


Timeline:

  • As few as 12 weeks for 1,000 glycerol stock clones


Retroviral vectors are also available

 
 

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Custom shRNA arrayed library workflow