Overexpression Stable Cell Lines  


High-quality stable cell line generation from LentiORF Select-Vectors™


Transomic offers overexpression stable cell lines generated from LentiORF Select-VectorsTM.  Our extensive collection of constitutive and inducible lentiviral vectors allows for customization of the target gene expression cassette to include the appropriate selectable markers and tags for the specific experiment.

 


Workflow:

  • Choose a lentiviral vector
  • Provide the gene of interest and sequence or Genbank accession number
  • Select the preferred cell line
  • A mock vector map is provided for review and final approval before synthesis and cloning of the gene of interest
  • The LentiORF Select-Vector clone is sequence-verified, viral particles are packaged, and the selected cell line is infected


Deliverable:

  • Polyclonal or monoclonal overexpression cell populations
  • QC data that includes Western Blot and/or qPCR data (sequencing and flow cytometry also available)
  • Glycerol stock, plasmid DNA, and/or lentiviral particles of the LentiORF Select-Vector clone
  • Stability testing is available


Timeline:

  • Polyclonal cell line is delivered in as few as 8 weeks

 

Click to view available LentiORF Select-Vectors™
 

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CHO cells infected with LentiORF Select-Vector containing Gene X.
Single Cells were isolated and expanded to produce a monoclonal
cell line.
Expression levels of target protein are approximately
100x fold that
of the parental line.