MGC premier cDNA and ORF Clones

Full-Length, fully-sequenced cDNA clones... the gold standard for 20+ years

MGC premier cDNA and ORF collections offer a convenient solution that decreases the time required to start an experiment and increases the chances of success. The Mammalian Gene Collection (MGC) was created though an NIH initiative involving several labs to create the most comprehensive cDNA and ORF libraries available to the scientific community. All cDNA and ORF information can be researched through NCBI Genbank and can be conveniently ordered through Transomic’s gene search tool.

Advantages

MGC clones have been used by research labs around the globe for the past 20+ years
Quick delivery of glycerol stock cDNA and ORF clones
Subset of LentiORF clones in the V5-tagged, pLX304 overexpression lentiviral vector
Subset of clones in mammalian expression vectors with CMV promoter
Subset of clones available that are Gateway™ compatible
Since it is best practice to sequence-confirm all clones before using them in research applications, Transomic offers a sequence-confirmation service for your clone. We will end-sequence the cDNA or ORF clone and BLAST-confirm the sequence to Genbank.

Workflow

Enter your gene of interest into our gene search tool (gene symbol, gene ID, Genbank accession #)
Use the filter on the left of the screen to select cDNA/ORF
Choose the cDNA or ORF clone that best suits your experiment
If optional end-sequencing service is needed, just choose the ‘end-sequence before shipping’ version in the gene search results.

Deliverable

Glycerol stock in 2ml tube
Custom arrays of cDNA/ORF clones are available
End-sequence BLAST report when selecting ‘end-sequence before shipping’

Timeline

48 hours for standard glycerol stock clones. 1-2 weeks for the end-sequence confirmation option

Price

$140 for standard glycerol stocks. $175 for end-sequence confirmation option

To place your order, go to the gene search tool at the top of the page and enter
your gene of interest.

MGC premier cDNA and ORF Library creation graphic

Access Publication

References:

MGC Premier human and mouse cDNA libraries

Temple, Gary, et al. The completion of the mammalian gene collection (MGC). Genome Research 19(12), 2324-2333 (2009). https://genome.cshlp.org/content/19/12/2324.short
MGC Website: https://genecollections.nci.nih.gov/MGC/

MGC Premier human ORFeome Collaboration Library

Wiemann, S., Pennacchio, C., Hu, Y. et al. The ORFeome Collaboration: a genome-scale human ORF-clone resource. Nat Methods 13, 191–192 (2016). https://doi.org/10.1038/nmeth.3776
ORFeome Collaboration Website: http://www.orfeomecollaboration.org/

MGC Premier human ORFeome v8.1 Library

Yang, X., Boehm, J., Yang, X. et al. A public genome-scale lentiviral expression library of human ORFs. Nat Methods 8, 659–661 (2011). https://doi.org/10.1038/nmeth.1638
CCSB hORFeome Database (Dana Farber Cancer Institute): http://horfdb.dfci.harvard.edu/index.php?page=home
Broad Institute hORFeome v8.1: https://www.broadinstitute.org/scientific-community/science/platforms/gpp/horfeome-v81-library

MGC premier Lentiviral ORF Expression Library

Yang, X., Boehm, J., Yang, X. et al. A public genome-scale lentiviral expression library of human ORFs. Nat Methods 8, 659–661 (2011). https://doi.org/10.1038/nmeth.1638

Related Products and Services

LentiORF Select-Vectors™
Choose the ideal lentiviral vector for your experiment. Full catalog of lentiviral vectors to choose from. Select glycerol stock format or ready-to-use lentiviral particles.

Custom Gene Synthesis

Transomic will synthesize your specific insert sequence and clone it into the LentiORF Select-Vector™ that best suits your experiment. We can also clone into a vector provided to us by your lab.

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

cDNA and ORF Whole Genome Arrayed Libraries

cDNA and ORF arrayed libraries are great resources for core labs. Choose the collection(s) that best suit your needs…Lentiviral ORF Expression vector library, mammalian expression vector libraries, or Gateway™ compatible entry ORF libraries.