shERWOOD-UltramiR shRNA

shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown

shERWOOD-UltramiR shRNA reagents are next generation vector-based RNAi triggers designed using the proprietary shERWOOD algorithm developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory (Knott et al 2014). An alternate microRNA scaffold "UltramiR" has been optimized for increased shRNA processing and potency based on new information on the key determinants for primary microRNA processing (Auyeung et al 2013).

The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome. The shERWOOD algorithm designs have been applied to the creation of  shERWOOD-UltramiR shRNA libraries targeting human, mouse and rat genomes.


  •     shERWOOD algorithm designs - superior knockdown performance relative to existing shRNA designs
  •     UltramiR scaffold - increases shRNA processing and potency
  •     All transcripts for a gene are targeted for the most complete knockdown
  •     Whole Genome arrayed library and Focused subset arrayed libraries are available
  •     Custom screening pools are available (go to pools)



Available vectors for your gene of interest

Lentiviral vectors


Inducible lentiviral vector


Retroviral vectors



Unsure of the best promoter to use for your specific cell line?
Use our Promoter Selection Kit on your cells prior to ordering your shRNA gene sets.

Learn more about the promoter selection kit

Promoter Selection Kit for shRNA knockdown


Supporting Data
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