RNAi Controls

shERWOOD-UltramiR shRNA Negative Controls

Including high-quality and proper controls is critical to a gene silencing experiment. They enable accurate interpretation of knockdown data and provide confidence in the specificity of the response. Lentiviral shRNA controls are available as lentiviral particles or bacterial glycerol stock controls. Retroviral shRNA controls are available as a bacterial glycerol stock.

Advantages

Changes in the mRNA or protein levels in cells treated with non-targeting controls reflect non-specific responses and can be used as a baseline against which specific knockdown can be measured. The non-targeting controls below do not target any mammalian genes.

Available Vectors

Lentiviral vectors

pZIP

Inducible lentiviral vector

pZIP-TRE3G

Retroviral vectors

pLMN

pLMP-d

Timeline

Typically ships within 48 hours of order receipt for glycerol stock format. Lentiviral particle format will take additional time.

Select your format, promoter and reporter for your negative control and add to cart to purchase.

shERWOOD-UltramiR Lentiviral shRNA Negative Controls

SELECT :

Format

Promoter

Selection Marker

Fluorescent Reporter

shERWOOD-UltramiR Lentiviral shRNA Negative Controls $745.00

shERWOOD-UltramiR Inducible Lentiviral shRNA Negative Controls

SELECT :

Format

Selection Marker

Fluorescent Reporter

shERWOOD-UltramiR Inducible Lentiviral shRNA Negative Controls $330.00

shERWOOD-UltramiR Retroviral shRNA Negative Controls

SELECT :

Vector

shERWOOD-UltramiR Retroviral shRNA Negative Controls $330.00

Related Products and Services

shRNA Whole Genome Arrayed Libraries (Human and Mouse)

Transomic’s arrayed shRNA libraries are a great resource for core labs. Arrayed libraries are offered as glycerol stocks in 96-well plates. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Custom shRNA Pools and Arrayed Libraries

Transomic can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process. All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner.

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

Custom shRNA Vector Construction Service

Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.

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