shRNA Target Gene Sets  


Target your gene of interest with potent shERWOOD-UltramiR™ shRNA constructs

 

Select the ideal lentiviral or retroviral vector for your gene knockdown experiment. Transomic offers complete flexibility with a portfolio of vectors containing different promoter options (including dox inducible options), selection markers, and fluorescent reporters. Simply search for your gene of interest using our online tool to make your vector choice. You will receive 3 unique shRNA constructs plus a negative control. All shRNAs are designed using the shERWOOD algorithm.
 
The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.
 


Advantages

 
  • Unmatched shERWOOD algorithm for effective shRNA designs
  • UltramiR miR-30 scaffold increases potency
  • Dox inducible lentiviral vectors available


Workflow

  • Enter your gene of interest into our gene search tool (gene symbol, gene id, Genbank accession #)
  • Use the filter on the left of the screen to select shRNA
  • Select the lentiviral or retroviral vector option that best suits your experiment
  • Choose the format to be delivered (glycerol stock or ready-to-use lentiviral particles)


Deliverable

  • 3 shRNA constructs targeting your gene of interest plus a negative control
  • 100% sequence-confirmed shRNA constructs
  • Glycerol stocks or lentiviral particles
  • Plasmid DNA can be delivered upon request as a custom quote

Timeline
  • 2-3 weeks for glycerol stocks. Additional 2 weeks for viral particles

Price
  • Starting at $695 for glycerol stocks and $2,695 for concentrated lentiviral particles
 

Available shRNA vectors for your gene of interest


Lentiviral vectors

pZIP



Inducible lentiviral vector

pZIP-TRE3G


Retroviral vectors

pLMN


pLMP-d
 


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To place your order, go to the gene search tool at the top of the page and enter
your gene of interest.

 
Transomic gene search tool - Search your gene of interest to find CRISPR products

Related Products and Services

 

Transomic’s arrayed shRNA libraries are a great resource for core labs. Arrayed libraries are offered as glycerol stocks in 96-well plates. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Transomic can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process. All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner. 

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.  This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
 
Transomic has a portfolio of CRISRP vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal TRACR, promoter, selection marker, or fluorescent reporter for your application.
 
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