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Transomic’s arrayed shRNA libraries are a great resource for core labs. Arrayed libraries are offered as glycerol stocks in 96-well plates. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.
Transomic can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process. All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner.
Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
Transomic has a portfolio of shRNA vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal promoter, selection marker, or fluorescent reporter for your application.
shRNA negative controls enable accurate interpretation of knockdown data and provide confidence in the specificity of the response. Lentiviral shRNA and Inducible lentiviral shRNA controls are available as lentiviral particles or bacterial glycerol stock. Retroviral shRNA controls are available as bacterial glycerol stock.
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