shRNA Target Gene Sets

Target your gene of interest with potent shERWOOD-UltramiR™ shRNA constructs

Select the ideal lentiviral or retroviral vector for your gene knockdown experiment. Transomic offers complete flexibility with a portfolio of vectors containing different promoter options (including dox inducible options), selection markers, and fluorescent reporters. Simply search for your gene of interest using our online tool to make your vector choice. You will receive 3 unique shRNA constructs plus a negative control. All shRNAs are designed using the shERWOOD algorithm.

The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Advantages

Unmatched shERWOOD algorithm for effective shRNA designs
UltramiR miR-30 scaffold increases potency
Dox inducible lentiviral vectors available

Workflow

Enter your gene of interest into our gene search tool (gene symbol, gene id, Genbank accession #)
Use the filter on the left of the screen to select shRNA
Select the lentiviral or retroviral vector option that best suits your experiment
Choose the format to be delivered (glycerol stock or ready-to-use lentiviral particles)

Deliverable

3 shRNA constructs targeting your gene of interest plus a negative control
100% sequence-confirmed shRNA constructs
Glycerol stocks or lentiviral particles
Plasmid DNA can be delivered upon request as a custom quote

Timeline

2-3 weeks for glycerol stocks. Additional 2 weeks for viral particles

Price

Starting at $820 for glycerol stocks and $2,965 for concentrated lentiviral particles

Available shRNA vectors for your gene of interest

Lentiviral vectors

pZIP

Inducible lentiviral vector

pZIP-TRE3G

Retroviral vectors

pLMN

pLMP-d

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To place your order, go to the gene search tool at the top of the page and enter
your gene of interest.

Transomic gene search tool - Search your gene of interest to find CRISPR products

Related Products and Services

shRNA Whole Genome Arrayed Libraries (Human and Mouse)

Transomic’s arrayed shRNA libraries are a great resource for core labs. Arrayed libraries are offered as glycerol stocks in 96-well plates. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Custom shRNA Pools and Arrayed Libraries

Transomic can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process. All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner.

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

Custom shRNA Vector Construction Service

Transomic has a portfolio of shRNA vector backbones that can be used to tailor the best vector for your experiment. Choose the ideal promoter, selection marker, or fluorescent reporter for your application.

RNAi Controls

shRNA negative controls enable accurate interpretation of knockdown data and provide confidence in the specificity of the response. Lentiviral shRNA and Inducible lentiviral shRNA controls are available as lentiviral particles or bacterial glycerol stock. Retroviral shRNA controls are available as bacterial glycerol stock.

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