shRNA Whole Genome Arrayed Library


shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown

 

Transomic’s shERWOOD-UltramiR™ shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory
(Knott et al 2014). The library uses an alternate microRNA scaffold called "UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).

 

The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

 

Features

 
  • Over 18,000 human and mouse genes targeted
  • Each human gene is targeted by an average of 6 shRNA constructs and each mouse gene is targeted by an average of 5 shRNA constructs
  • The shRNAs are cloned into a retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter

Retroviral shRNA vector - pLMN
 

Advantages
  • The proprietary shERWOOD algorithm was used to design all shRNA. Read the publication in Molecular Cell
  • The UltramiR microRNA scaffold increases shRNA processing and potency
  • All human and mouse shRNA library constructs are 100% sequence-confirmed
  • Glycerol stock 96-well plates are easily maintained and offer a convenient workflow

Deliverables
  • Human shRNA library: 1,125/96-well glycerol stock plates with barcoded labels
  • Mouse shRNA library: 898/96-well glycerol stock plates with barcoded labels
  • Datafile for 96-well plate clone mapping

Timeline
  • 4-6 weeks upon receipt of order

 

Price
  • Contact us for a quote. Academic pricing available


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Related Products and Services
 
Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique shRNA constructs  plus a negative control. All shRNA constructs are 100% sequence-confirmed before shipping.
 
Transomic offers custom shRNA pooled libraries for screening projects and genome editing experiments. All constructs are cherry-picked from our arrayed, sequence-verified shRNA libraries and can be subcloned into the vector of choice.
 
Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.  This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.
 
Lentiviral and retroviral shRNA cloning vectors are delivered as glycerol stocks for an unlimited supply for cloning experiments.
 

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