shRNA Whole Genome Arrayed Library

shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown

Transomic’s shERWOOD-UltramiR™ shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory
(Knott et al 2014). The library uses an alternate microRNA scaffold called "UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).

The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Features

Over 18,000 human and mouse genes targeted
Each human gene is targeted by an average of 6 shRNA constructs and each mouse gene is targeted by an average of 5 shRNA constructs
The shRNAs are cloned into a retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter

Advantages

The proprietary shERWOOD algorithm was used to design all shRNA. Read the publication in Molecular Cell
The UltramiR microRNA scaffold increases shRNA processing and potency
All human and mouse shRNA library constructs are 100% sequence-confirmed
Glycerol stock 96-well plates are easily maintained and offer a convenient workflow

Deliverables

Human shRNA library: 1,125/96-well glycerol stock plates with barcoded labels
Mouse shRNA library: 898/96-well glycerol stock plates with barcoded labels
Datafile for 96-well plate clone mapping

Timeline

4-6 weeks upon receipt of order

Price

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shERWOOD shRNA Algorithm screen selection

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Related Products and Services

shRNA Target Gene Sets

Search for your gene of interest using our simple online tool that allows customization for gene modulation type, vector features, and deliverable format. You will receive 3 unique shRNA constructs plus a negative control. All shRNA constructs are 100% sequence-confirmed before shipping.

Custom shRNA Screening Pools

Transomic offers custom shRNA pooled libraries for screening projects and genome editing experiments. All constructs are cherry-picked from our arrayed, sequence-verified shRNA libraries and can be subcloned into the vector of choice.

Custom Lentiviral Packaging Services

Transomic’s lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction. Refer to our product guides for best practices.

shRNA Cloning Vectors

Lentiviral and retroviral shRNA cloning vectors are delivered as glycerol stocks for an unlimited supply for cloning experiments.

RNAi Controls

shRNA negative controls enable accurate interpretation of knockdown data and provide confidence in the specificity of the response. Lentiviral shRNA and Inducible lentiviral shRNA controls are available as lentiviral particles or bacterial glycerol stock. Retroviral shRNA controls are available as bacterial glycerol stock.

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