Yeast Knockout Collection

Pooled Screening Libraries

Now available in a pooled format for simultaneous analysis of large numbers of deletion strains through selective growth conditions. Identification and quantitation of the deletions surviving selection can be determined by hybridizing the deletions to a microarray containing all the barcode oligos. The barcode oligos are unique to each deletion, allowing rapid, high-throughput identification of the deletions. Each Yeast Deletion Pool contains all the deletions within the strain, equally represented within the pool. Yeast Deletion Pools are supplied in ten tubes, each containing 500 µl of the pooled deletions.

References:

1. Shoemaker, DD. et al. (1996). Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy. Nat Genet. 1996 Dec;14(4):450-6.
2. Saccharomyces Genome Deletion Project

Schematic of homologous recombination between the barcoded deletion cassette and the yeast chromosomal DNA.  (Graphics adapted from publication: The uses of genome-wide yeast mutant collections: Bart Scherens and Andre Goffeau,  Genome Biology 2004, 5:229.)

Deletion Strain Creation

Figure 1:  Schematic of barcode screening analysis showing differential selection of yeast deletion strains and readout on microarray.  (Graphics adapted from publication: The uses of genome-wide yeast mutant collections: Bart Scherens and Andre Goffeau,  Genome Biology 2004, 5:229.) 

High Throughput Parallel Screening

References

1. Giaever G, Chu AM, Ni L, Connelly C, Riles L, Veronneau S, Dow S, Lucau-Danila A, Anderson K, Andre B, et al.: Functional profiling of the Saccharomyces cerevisiae genome. Nature 2002, 418:387-391.
2. Bart Scherens and Andre Goffeau:  The uses of genome-wide yeast mutant collections. Genome Biology 2004, 5:229