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Yeast Knockout Collection



In 1996 S. cerevisiae became the first eukaryotic genome to be fully-sequenced, enabling the creation of the first molecular-barcoded, genome-wide yeast knockout collection (1, 2).  The collection includes over 20,000 deletion strains corresponding to 5,916 genes (including 1,159 essential genes).  
Each strain has a complete start-codon to stop-codon deletion of an open reading frame (ORF) that is flanked by two molecular barcodes.  This collection has been used successfully in a number of screens and has been cited over 780 times (3).

Advantages of the collection include:
  • Molecular barcodes allow high-throughput parallel screening of entire genome
  • Complete deletions that ensure no residual gene function
  • Homozygous and heterozygous formats ready for screening
  • Haploid collections can be crossed to cell based assay strains to expand screening options
The following collections are available as individual strains, custom rearrays and pooled screening libraries.
  • Yeast Homozygous Diploid Collection - Genome-wide screening under various conditions
  • Yeast Heterozygous Diploid Collection - Screening for haploid sufficient genetic interactions
  • Yeast MAT-a or MAT-alpha Haploid Collections - Cross to other strains expanding options for screening assays
  • Yeast Essential Collection - Targeting genes essential for growth on rich media

 

Pooled Screening Libraries

Now available in a pooled format for simultaneous analysis of large numbers of deletion strains through selective growth conditions. Identification and quantitation of the deletions surviving selection can be determined by hybridizing the deletions to a microarray containing all the barcode oligos. The barcode oligos are unique to each deletion, allowing rapid, high-throughput identification of the deletions. Each Yeast Deletion Pool contains all the deletions within the strain, equally represented within the pool. Yeast Deletion Pools are supplied in ten tubes, each containing 500 µl of the pooled deletions.

Yeast Knockout (YKO) Collection

 

Schematic of homologous recombination between the barcoded deletion cassette and the yeast chromosomal DNA.  (Graphics adapted from publication: The uses of genome-wide yeast mutant collections: Bart Scherens and Andre Goffeau,  Genome Biology 2004, 5:229.)

Deletion Strain Creation

The Yeast Knockout (YKO) Collection was created by systematically targeting every open reading frame (ORF) and creating a start- to stop-codon deletion.  The cassette used to delete the ORF consists of a selectable marker (kanamycin-resistance KanMX4) flanked by two barcode sequences.  These are referred to as the UPTAG and DOWNTAG and are unique to each gene.  The 5' and 3' sequences of the cassette are homologous to the targeted gene to allow recombination.  Following recombination, the selection marker replaces the ORF and the barcodes are integrated into the genome.

 

Figure 1:  Schematic of barcode screening analysis showing differential selection of yeast deletion strains and readout on microarray.  (Graphics adapted from publication: The uses of genome-wide yeast mutant collections: Bart Scherens and Andre Goffeau,  Genome Biology 2004, 5:229.) 

High Throughput Parallel Screening

The UPTAG and DOWNTAG barcodes (Figure 1) allow high-throughput parallel screening.  Starting with a population where each yeast deletion strain is represented equally, selective pressure can be applied.  Strains resistant to the selection become more highly represented than sensitive strains during growth of the culture.  The changes in population correlate with changes in the barcode representation and can be analyzed by microarray or next generation sequencing.

The selective pressure is determined by the type of screen.  One of the initial validations of the yeast knockout collection highlights its utility and flexibility by performing a number of screens using selection for growth in high salinity, low salinity, high pH, changes in carbon source and peroxide stress1.  There have been a number of screens since including, but not limited to, examining exposure to ionizing radiation, DNA damaging reagents and defects in cell division2.
Catalog # Description Price Buy
TKY3504P Yeast Knockout Collection Essential Genes; Pool of 10 x 0.5ml (glycerol stock) $610.00
TKY3500P Yeast Knockout Collection Homozygous Diploid; Pool of 10 x 0.5ml (glycerol stock) $5,200.00
TKY3501P Yeast Knockout Collection Heterozygous Diploid; Pool of 10 x 0.5ml (glycerol stock) $5,200.00
TKY3502P Yeast Knockout Collection Haploid Mat-a; Pool of 10 x 0.5ml (glycerol stock) $5,200.00
TKY3503P Yeast Knockout Collection Haploid Mat-alpha; Pool of 10 x 0.5ml (glycerol stock) $5,200.00
Catalog # Description Price Buy
TKY3500 Yeast Deletion Collection Homozygous Diploid $6,400.00
TKY3501 Yeast Deletion Collection Heterozygous Diploid $4,000.00
TKY3502 Yeast Deletion Collection Haploid Mat-a $6,400.00
TKY3503 Yeast Deletion Collection Haploid Mat-alpha $6,400.00
TKY3504 Yeast Deletion Collection Essential Genes $1,500.00